Search results for "Molecular characterisation"
showing 5 items of 5 documents
Cultivable microorganisms associated with honeys of different geographical and botanical origin
2014
In this study, the composition of the cultivable microbial populations of 38 nectar honey and honeydew honey samples of different botanical and geographical origin were assessed. After growth in specific media, various colonies with different appearance were isolated and purified before phenotypic (morphological, physiological and biochemical traits) and genotypic [randomly amplified polymorphic DNA (RAPD), repetitive DNA elements-PCR (rep-PCR) and restriction fragment length polymorphism (RFLP)] differentiation. The identification was carried out by 16S rRNA gene sequencing for bacteria and, in addition to RFLP, by sequencing the D1/D2 region of the 26S rRNA gene for yeasts and the 5.8S-IT…
Technical Note on the quality of DNA sequencing for the molecular characterisation of genetically modified plants
2018
Abstract As part of the risk assessment (RA) requirements for genetically modified (GM) plants, according to Regulation (EU) No 503/2013 and the EFSA guidance on the RA of food and feed from GM plants (EFSA GMO Panel, 2011), applicants need to perform a molecular characterisation of the DNA sequences inserted in the GM plant genome. The European Commission has mandated EFSA to develop a technical note to the applicants on, and checking of, the quality of the methodology, analysis and reporting covering complete sequencing of the insert and flanking regions, insertion site analysis of the GM event, and generational stability and integrity. This Technical Note puts together requirements and r…
Human and animal fascioliasis in andean regions of argentina: lymnaeid vectors and livestock reservoirs
2018
La fascioliasis es una enfermedad parasitaria zoonótica causada en América por Fasciola hepatica. Produce serias pérdidas económicas a la ganadería y es un importante problema en salud pública a nivel mundial. Las más altas prevalencias en humanos se hallan en regiones andinas. La especie de lymnaeido vector involucrada condiciona el escenario epidemiológico. En Argentina existía gran controversia acerca de las especies de lymnaeidos vectores presentes. Además de los bovinos, escasos estudios han considerado el rol que cumplirían otras especies domésticas y silvestres como reservorios. La fascioliasis humana ha sido considerada una enfermedad de escasa relevancia. El objetivo del presente t…
Influence of biotic and abiotic factors on soil organic matter dynamics assessed by the biogeochemical characterisation of soluble organic matter
2014
Soils are the greatest reservoir of C on the continents, and organic matter mineralisation bymicrobial activity represents the major part of the CO2 emitted by terrestrial ecosystems.In this work, we studied water-extractable organic matter (WEOM), which corresponds to themore reactive fraction of soil organic matter (SOM). Our objectives were (i) to identify therelationships of WEOM dynamics with bacterial communities, and with soil physico-chemicalparameters; (ii) to provide a precise chemical characterisation of WEOM.There is a strong link between SOM solubility and the structure of bacterial communities, andan erosion of their diversity has an impact on SOM and WEOM dynamics, and leads …
Fasciolasis humana y animal en África, con enfásis en Egipto
2013
Fasciolid flukes cause fascioliasis, an helminthic disease of humans and livestock and which is transmitted by specific freshwater snails of the family Lymnaeidae, within a frame of transmission and epidemiology pronouncedly influenced by climate factors and environmental conditions. In Africa, fascioliasis is caused by the two species Fasciola hepatica and F. gigantica, giving rise to important economic losses in husbandry throughout. Research on human infection has focused on northern Maghreb countries and Egypt, whereas studies on the wide region southward from the Sahara have only been sporadic. The present Thesis includes the genetic characterisation of the lymnaeid species found in se…